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Cell cycle-specific oscillations of cyclin E are conserved in endoreplicating cells. (A) Normally cycling HT2-19 cells were arrested in S phase with HU and released into fresh medium. Cells were harvested at the indicated times and analyzed by immunobloting with cyclin E, actin, and cdk1 antibodies, as well as by FACS. (B) Cells were deprived of IPTG for 7 d to deplete their cdk1 and were subsequently arrested with HU, released and analyzed like in A.
