Figure 1.

Identification of a novel Rab7 target, Rabring7. (A) Predicted amino acid sequence of Rabring7. The amino acid sequence deduced from the nucleotide sequence of the full-length Rabring7 cDNA is shown in single letter code. The underline shows the clone 7-3-18 encoded region. The box indicates the RING finger motif and the shaded boxes indicate conserved amino acids in the RING finger motif. (B) Specific interaction between Sos-Rab7Q67LΔC and clone 7-3-18 in the CytoTrap system. Yeast strain cdc25Hα were transformed with the indicated plasmids and the transformants were assayed for growth as described in MATERIALS AND METHODS. (C) Northern blot analysis. Mouse multiple tissue blots (Clontech) were hybridized with Rabring7 cDNA probe (top) or GAPDH cDNA probe (bottom).