Figure 6.

RAM protein interaction network. (A) Pairwise two-hybrid qualitative LacZ assays were performed among the various RAM proteins. The corresponding amino acid regions of each protein are noted in parentheses. Red boxes indicate a positive interaction; black boxes indicate no interaction. (B) Coimmunoprecipitation of Kic1-HA with Hym1-Myc. Extracts prepared from cells expressing Hym1-Myc or untagged Hym1p were immunoprecipitated with anti-Myc. Immunoprecipitated proteins were detected by immunoblot analysis with antibodies directed against HA (top) or Myc (bottom). Strains used were Kic1-HA Hym1-Myc (Y4089) and Kic1-HA (Y3615). (C) Summary of the RAM protein interaction network based on our data and that of others (Racki et al., 2000; Ito et al., 2001; Du and Novick, 2002; Ho et al., 2002; Weiss et al., 2002). YOR353c = SOG2.