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. 1998 Mar 17;95(6):2844–2849. doi: 10.1073/pnas.95.6.2844

Figure 2.

Figure 2

Gel mobility-shift assays for DNA binding. C. elegans AHR-1 and AHA-1 and mammalian AHR and ARNT were transcribed and translated in vitro in independent reactions, combined as indicated in the presence or absence of β-naphthoflavone (βNF), and incubated with a labeled XRE probe. The reactions were then electrophoresed on nondenaturing polyacrylamide gels. Unlabeled XRE or XREmut2 DNA was added as competitor to some reactions to assay the sequence specificity of binding. In (C), an additional 2 μl (+++) and 0.5 μl (++) of baculovirus-expressed ARNT (42) was added as indicated to the binding reactions. Arrows indicate the position of free probe.