Figure 3.
Coimmunoprecipitation with HSP90. C. elegans AHR-1 and AHA-1 and mammalian AHR and ARNT were independently translated and labeled with [35S]methionine in rabbit reticulocyte lysates. Rabbit HSP90 antibody or preimmune sera were used to immunoprecipitate (IP) proteins from these reactions, and the HSP90-associated proteins were resolved by SDS/PAGE. The percentage of total protein translated that immunoprecipitated was calculated, and the average of two experiments was recorded as “% IP”.