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. 1998 Mar 17;95(6):2844–2849. doi: 10.1073/pnas.95.6.2844

Figure 5.

Figure 5

Receptor activation assays in yeast. (A) Schematic representation of C. elegans AHR-1 and of the LexA protein fusions expressed by pJ350Y, pJ353Y, and the LexA-AHRNΔ166 control construct (29). In pJ350Y, the LexA DNA binding and dimerization domains replace the AHR-1 bHLH domain. pJ353Y lacks the PAS domains. The control construct LexA-AHRNΔ166 is a fusion of LexA and human AHR as diagrammed. The regions of the AHR PAS domain required for binding to HSP90 and to ligand (13) are indicated. (B) β-naphthoflavone (βNF) dose-response curves for the proteins encoded by pJ350Y and LexA-AHRNΔ166 in the yeast expression system. Immunoblot analyses (not shown) using anti-LexA antibody showed that similar amounts of the fusion proteins were synthesized in cells containing pJ350Y or LexA-AHRNΔ116.