Table 1.
Living cell analysis of the length (mean ± s.d.) in minutes of different stages of mitosis in PtK1 cells progressing through mitosis in TSA-containing medium
| N | Prometaphase | Metaphase | Anaphase | Telophase/Cytokinesis | Total | |
|---|---|---|---|---|---|---|
| 5 h DMSO | 29 | 18.8 ± 9.4 | 21.6 ± 11.6 | 17.1 ± 6.2 | 17.8 ± 9.6 | 75.3 ± 25.1 |
| 30 min TSA | 30 | 26.9 ± 17.9a | 24.4 ± 21.5 | 15.1 ± 7.6 | 22.0 ± 16.3 | 87.5 ± 38.6c |
| 5 h TSA | 29 | 40.4 ± 15.9b | 27.8 ± 10.8a | 18.7 ± 8.4 | 24.4 ± 16.0 | 108.9 ± 20.9bd |
Cells were exposed to TSA for the indicated times before observation and kept in TSA-containing medium during the experiment. N = total number of cells analyzed.
a
Student t test, p < 0.05; b, Student t test, p < 0.01, when values were compared to DMSO treatment.
c
1 cell: prometaphase arrest (> 3 h); 1 cell: metaphase arrest (> 3 h); 3 cells: absence of cytokinesis.
d
6 cells: prometaphase arrest (> 3 h); 2 cells: absence of cytokinesis.