Figure 4.
Effects of E2F-6 on transactivation of an E2F4-CAT reporter C33-A cells were transiently transfected in duplicate with 4 μg of E2F4-CAT, 2 μg of Rous sarcoma virus–luciferase (as an internal control), 14 μg of carrier DNA (pBKS+ and pCMV-neo-Bam) in the absence or presence of 3 μg of pCMV-HA-DP2 plus pCMV-E2F expression vectors as indicated. CAT and luciferase activity were determined 24 h after transfection. The values shown are the average of duplicate transfectants for representative experiments.