Table I.

Enzyme activity determination in leaves and green fruits of wild type and hexokinase over-expressing plants

Single leaf or fruit samples of six plants were measured. Leaves were harvested six h into the light period from fully-expanded mature leaves of six-week-old plants. Green fruit samples were taken at 30 d after flowering. Values are presented as the mean ± se. Values that were significantly different from WT are set in bold. AGPase, ADPglucose pyrophosphorylase; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; PFK, Phosphofructokinase; PGI, Phosphoglucose isomerase; PGM, Phosphoglucomutase; TPI, triose-phosphate isomerase; ^ϕ for PGM in leaves and TPI in fruit activities are presented in μmol min⁻¹ g fresh wt⁻¹.

Enzyme	Wild-Type	HK4	HK37	HK38
	nmol min−1 g fresh wt−1
Leaf activity
AGPase	437 ± 24	421 ± 64	477 ± 51	434 ± 54
Aldolase	6,728 ± 252	4,287 ± 291	6,631 ± 401	5,689 ± 510
Enolase	623 ± 76	777 ± 100	447 ± 136	476 ± 109
Fructokinase	227 ± 61	2,029 ± 155	109 ± 31	879 ± 124
PGI	6,070 ± 282	6,766 ± 362	6,008 ± 290	5,491 ± 661
PGMϕ	12.6 ± 0.3	13.9 ± 0.4	10.8 ± 0.6	11.7 ± 0.9
Pyruvate kinase	6,021 ± 392	4,938 ± 325	5,115 ± 212	5,272 ± 474
PFK	345 ± 41	485 ± 67	346 ± 33	283 ± 26
GAPdH	8,789 ± 647	5,126 ± 547	6,421 ± 309	6,441 ± 832
TPI	9,560 ± 375	8,152 ± 476	9,436 ± 406	9,193 ± 891
Fruit activity
AGPase	21 ± 5	12 ± 1	19 ± 1	21 ± 1
Aldolase	264 ± 13	290 ± 18	343 ± 16	501 ± 37
Enolase	200 ± 79	212 ± 29	256 ± 40	231 ± 3
Fructokinase	136 ± 16	232 ± 41	303 ± 32	398 ± 24
PGI	32 ± 7	51 ± 11	55 ± 3	15 ± 4
PGM	580 ± 66	765 ± 51	782 ± 46	969 ± 46
Pyruvate kinase	407 ± 23	643 ± 67	673 ± 74	1,091 ± 56
PFK	152 ± 19	155 ± 11	231 ± 14	237 ± 22
GAPdH	39 ± 5	42 ± 6	81 ± 22	110 ± 28
TPIϕ	12.4 ± 2.0	12.3 ± 0.2	11.7 ± 0.5	12.3 ± 0.0