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. 2003 Sep;133(1):100–112. doi: 10.1104/pp.103.023358

Figure 5.

Figure 5.

arl2-2/At2g20050 recombinant DNA fragments can be PCR amplified from arl2-2 genomic DNA but not from wild-type DNA. Primers used to PCR amplify wild-type ARL2 or recombinant arl2-2/At2g20050 fragments from Ws or arl2-2 DNAs are shown by horizontal arrowheads on the diagram shown in Figure 4B and marked F1 (PP2C-F1), R2 (PP2C-R2), XR2, and R7 (ARL2-R7) on that diagram and at the top of the gel. Their respective sequences are provided in Table I. The DNA used as a template in diagnostic PCR reactions is indicated above each electrophoretic lane. The sizes of amplified fragments, as determined by comparison with Mr markers loaded on the same gel, are shown on the right of the gel.