Figure 1.

Northern blot analysis of small, stable RNAs accumulated in exoribonuclease-deficient cells. Electrophoresis and Northern blot analysis were carried out as described in Experimental Procedures. Five micrograms of total RNA from each of the indicated strains was used except for the CCA⁻ strain in which less RNA was loaded. After electrophoresis, the bottom 10 cm of the gel, containing the RNA of interest, was transferred to a membrane and hybridized with a probe specific for the RNA. The lengths of RNA were determined by an adjacent DNA sequencing ladder. The identities of the RNAs examined are shown on the left. M indicates the size(s) of the mature RNA.