Figure 2.

Primer extension analysis of RNA from wild type and a RNase PH⁻T⁻D⁻BN⁻ mutant cell. The analysis was carried out as described in Experimental Procedures by using 20 μg of total RNA from each strain and 2 pmol of ³²P-labeled primers. The RNA analyzed is shown at the top. The mature 5′ end(s) is marked M, and the 5′ end of the nascent transcript are marked P. Unknown products are marked by X. Phage M13 mp18 DNA was used to generate the ladder.