Figure 2.
Time course of mimosine effect on protein synthesis. HeLa cells were treated with 600 μM mimosine for different times and then used for analysis of expression of p170 (A) and hPrt 1 (B) by Western blot, and global protein synthesis (C) by [35S]methionine incorporation. Actin was used as a loading control for Western blot. The relative expression levels of p170 in A and hPrt 1 in B were calculated based on the density of p170, hPrt 1 and actin bands measured using a Scion Image software.