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Effect of iron level on p170 expression. HeLa cells were cultured with or without 150 μM GC-7 (A), 200 μM DFO (B), or 400 μM ferric ammonium citrate in combination with mimosine (C) followed by determination of p170 level by using Western blot as described in MATERIALS AND METHODS. Actin was used as a loading control. The relative expression levels of p170 were calculated based on the density of p170 and actin bands measured using a Scion Image software.
