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. 2003 Sep;14(9):3942–3951. doi: 10.1091/mbc.E02-12-0784

Figure 7.

Figure 7.

Effect of mimosine and p170 on p27 expression. (A) Time course of the effect of mimosine on p27 expression. HeLa cells were treated with 600 μM mimosine for 0–8 h and then lysed for determination of p27 expression using Western blot. (B–E) Effect of p170 level on p27 expression (B and C) and synthesis (D and E). Down-regulating p170 expression in HeLa cells (A and C) and up-regulating p170 expression in NIH3T3 cells (B and D) were achieved by transient transfection with antisense (AS) and sense (S) p170 cDNA, respectively. The effect of the change of p170 level on p27 expression (B and C) and synthesis (D and E) was determined using Western blot and pulse labeling, respectively, as described in MATERIALS AND METHODS. Vector-transfected cells (Vec) were used as controls for transfection. Actin was used as a loading control for Western blot. The relative expression levels of p27 (A–C) were calculated based on the density of p27 and actin bands measured using a Scion Image software.