Mutations in the PetC and AtpD loci. a, In petc-1, the PetC gene (At4g03280) is disrupted by an insertion of the autonomous En transposon. The sequences of the empty donor sites of DNA from three independent somatic revertant leaves (REV1-3) were obtained by PCR; uppercase letters indicate plant DNA sequences flanking the En; insertion footprints left at each locus after En transposition are indicated by bold lowercase letters; and bold uppercase letters indicate the target site in the WT. In the other two mutant alleles, a copy of the nonautonomous Ds transposon (petc-2) and the ROK2 T-DNA (petc-3) are inserted 5′ of the start codon of the PetC gene. b, In atpd-1, a copy of the 5.2-kb SKI015 T-DNA is inserted in the promoter region of AtpD (At4g09650). PCR analysis indicated a small deletion of 7 bp adjacent to the right border of the SKI015 insertion. The En, Ds, and T-DNA insertions are not drawn to scale.