Figure 3.

AtpD and PetC mRNA and protein levels in WT and mutant plants. a, For northern analysis of the PetC transcript in petc-2 and WT plants, 30-μg samples of total RNA were analyzed using a fragment of the PetC transcript as a probe. A full-length AtpD cDNA was used as a probe for northern analysis of the AtpD transcript in atpd-1 and WT plants. As a loading control, the blots were reprobed with a cDNA fragment derived from the ACTIN1 gene. b, Samples of thylakoid membranes equivalent to 5 μg of Chl from WT and petc-2 or atpd-1 plants were fractionated by denaturing PAGE. Decreasing amounts of WT thylakoid membranes (3.75, 2.5, and 1.25 μg of Chl) were loaded in the lanes marked 0.75×, 0.5×, and 0.25× WT. Filters were probed with antibodies specific for the Rieske protein or the δ-subunit of the cpATPase.