Table 1.
Effect of various chemicals on steroid synthesis by C6 glioma and MA-10 Leydig cells
| Cell type | Treatment | P, fold increase | Progesterone | D | N (n) |
|---|---|---|---|---|---|
| C6 | Control | 1.0 | 1.0 | 1.0 | 4 (12) |
| FeSO4, 10 mM | 3.6 | 1.0 | 4.9 | 4 (18) | |
| FeCl3, 10 mM | 2.0 | 2.4 | 2.8 | 2 (4) | |
| H2O2, 10 mM | 2.0 | 1.0 | 2.0 | 1 (3) | |
| MA-10 | Control | 1.0 | 1.0 | 1.0 | 2 (5) |
| FeSO4, 10 mM | 1.2 | 1.0 | 2.0 | 2 (5) | |
| FeCl3, 10 mM | 1.1 | 1.0 | 1.8 | 2 (5) | |
| H2O2, 10 mM | 1.5 | 1.0 | 1.0 | 2 (5) |
Cell treatment, steroid isolation, and measurement were performed as described in Materials and Methods. Results shown are expressed as fold increase above control values. Basal values for C6 glioma cells are: P, 70 pg/mg protein; progesterone, 32 pg/mg protein; D, 4 pg/mg protein. Basal values for MA-10 Leydig cells are: P, 2,200 pg/mg protein; progesterone, 2,700 pg/mg protein; D, 7.4 pg/mg protein. N, number of experiments; n, number of samples.