Figure 2.

ABI4, ABI5, and CTR1 transcript accumulation is regulated by Glc. RT-PCR analysis of total RNA from 3-d-old wild-type (Wassilewskija [Ws]) seedlings grown in standard media and transferred to Murashige and Skoog 7% (w/v) Glc for 1, 3, 6, 24, and 72 h. The PCR product of APT1 was used as a cDNA loading control. Specific primers were used to amplify ABI4, ABI5, CTR1, and APT1 gene transcripts. The lengths of the PCR products are 974, 183, 353, and 478 bp for ABI4, ABI5, CTR1, and APT1, respectively. The linear phase of the exponential PCR reaction was corroborated for each gene (data not shown). A representative experiment from three biologically independent experiments is shown, including only the 24-h transference (T24) control for simplification. The means ± se of all three experiments and their corresponding transfer controls are included in Figure 4, D to F.