Figure 5.

Expression of Glu dehydrogenase (GDH) and Asn synthetase (AS) in nodules of transgenic L. japonicus expressing a GS1 antisense RNA in the nodules. The three T₁ generation plants are lines 5, 7, and 8, and the controls lines are Gifu and 21. Plants were grown for 4 weeks under symbiotic N-fixing conditions before harvest. A, NADH-dependent GDH activity. Values are the mean ± se of eight individual plants. Bars for the se are not presented when they are below the thickness of the line B. Western-blot analysis of GDH and AS proteins in nodules. Twenty micrograms of soluble protein was loaded into each lane. C, Northern-blot analysis of AS mRNA in nodules of LBC3-LjGS1As L. japonicus transformants (10 μg of total RNA was loaded into each lane and probed with a ³²P-labeled DNA fragment of the L. japonicus cDNA LJAS2; Waterhouse et al., 1996). Below is shown an ethidium bromide-stained gel to show equal RNA loading in all lanes.