Figure 2.

Tissue print analyses of tomato Chi9 and GluB mRNA expression in seeds of tomato hormone response or synthesis mutants and in response various elicitors. Chi9 (left panels) and GluB (right panels) mRNAs were assayed in seeds of the tomato genotypes indicated (wild-type, cv MM; GA-deficient mutant, gib-1; ethylene-insensitive mutant, nr; ABA-deficient mutant, sit^w) after imbibition for 48 h at 25°C in the indicated treatment solution (water; 100 μm ABA; 10 μL L^-1 ethylene, C₂H₄; 19 mm BABA; 10 μm FC; 100 μm MeJA; or 1 mm SA). Wild-type cv MM and gib-1 seeds served as positive and negative controls, respectively, for both genes. Prints of gib-1 seeds imbibed in GA are identical to those for cv MM seeds (data not shown). Four replicate tissue prints (as described in Fig. 1) are shown for each genotype or treatment.