Figure 4.

Tomato Chi9 and GluB expression in germinating seeds of the jasmonate-deficient defenseless1 (def1) mutant and its wild-type parent cv CM (A) or in wild-type cv MM leaves (B). A, Total RNA was extracted from seeds imbibed in water or in 100 μm MeJA for 24 h. B, Total RNA was isolated from leaves after the indicated times from treatment with water, 100 μm MeJA, or mechanical wounding. Equal amounts (10 μg) of RNA were loaded in each lane for hybridization analysis using Chi9 or GluB riboprobes.