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. 2003 Sep;133(1):287–294. doi: 10.1104/pp.103.026146

Figure 1.

Figure 1.

Organization of the transformed and nontransformed tobacco plastomes in the large single-copy region in the vicinity of rbcL. The LEV1 (Whitney et al., 1999) and LEV3 transformants have a promoter-less aadA gene inserted downstream of rbcL. For LEV1, the insertion point is downstream of the rbcL terminator sequence (T). For LEV3, the organization is similar, except that the rbcL terminator is deleted. The gene organization in tobacco-rubrum (tr) transformants is the same as for LEV3 transformants, except that the R. rubrum rbcM gene replaces rbcL (Whitney and Andrews, 2001a). The annealing positions of the probes, rbcL1, rbcL2, and aadA, and the primers, LsD and LsE, are shown. The positions of BamHI sites (B) and the sizes of the BamHI fragments that hybridize to the rbcL1 probe are indicated. The dashed arrows represent the various mono- and bicistronic rbc transcripts. H, HindIII; N, NcoI; P, rbcL promoter and 5′-untranslated region; t, rps16 terminator sequence; TSP, total soluble protein; nt; nontransformed.