Figure 6.

Kdo-8-P synthase gene expression in synchronized BY-2 cell suspensions. BY-2 cells were synchronized with aphidicolin (see `Materials and Methods'), and samples were taken every hour. A, Cell cycle progression monitored by flow cytometry and by measurement of the mitotic index (MI). B, Semiquantitative RT-PCR analysis of NtkdsA expression. Total RNA to be used for RT was isolated from the indicated samples in A. The RT-PCR analysis was performed as described in Figure 3. Specific cDNA probes were prepared for NtCDKA;1 (L77082), NtCDKB1;1 (AF289465), NtCycB1;1 (Z37978), NtCycD3;1 (AJ011893), and NtActin2 (U60490). C, Quantification of NtkdsA transcript accumulation. Signals obtained after autoradiography from B were quantified as described in Figure 3. The determination of relative NtkdsA mRNA abundance was normalized toward that of NtActin2.