Table I.
The specific activity of recombinant tomato Kdo-8-P synthase expressed in S. enterica sv. Typhimurium strains
| Strain
|
Growth
|
Specific Activitya
|
||||
|---|---|---|---|---|---|---|
| 30°C
|
42°C
|
Arabinose-5-P
|
Erythrose-4-P
|
Rib-5-P
|
||
| 30°C | 42°C | 42°C | 42°C | |||
| milliunits mg−1 protein | ||||||
| AG701 | + | + | 6.1 ± 0.2 | 18.7 ± 2.4 | ND | ND |
| AG701i50 | + | − | 1.3 ± 0.2 | 0.4 ± 0.1 | ND | ND |
| AG701-pBluescript | + | + | 5.2 ± 1.1 | 18.8 ± 1.5 | 0.99 ± 0.64 | 2.34 ± 0.35 |
| AG701i50-pBluescript | + | − | 1.2 ± 0.2 | 0.9 ± 0.2 | ND | ND |
| AG701-pLEKdoS | + | + | 16.4 ± 0.9 | 67.5 ± 2.8 | 0.67 ± 0.17 | 6.21 ± 0.42 |
| AG701i50-pLEKdoS | + | + | 60.5 ± 0.3 | 232.3 ± 11 | 0.83 ± 0.59 | 6.98 ± 1.07 |
| AG701-R61A/T62A | + | + | 1.3 ± 0.3 | 1,8 ± 0,5 | ND | ND |
| AG701i50-R61A/T62A | + | − | 0.7 ± 0.3 | 0.4 ± 0.1 | ND | ND |
| AG701-K136G | + | + | 3 ± 0.2 | 7.6 ± 0.8 | ND | ND |
| AG701i50-K136G | + | + | 1.2 ± 0.3 | 2.4 ± 0.5 | ND | ND |
| AG701-C164A | + | + | 9 ± 0.6 | 38.6 ± 4.3 | ND | ND |
| AG701i50-C164A | + | + | 7.4 ± 1.3 | 46 ± 8.9 | ND | ND |
| AG701-H198G | + | + | 5.3 ± 0.2 | 14.4 ± 0.5 | ND | ND |
| AG701i50-H198G | + | + | 0.5 ± 0.2 | 1.6 ± 0.4 | ND | ND |
| AG701-H242G | + | + | 3.7 ± 0.4 | 12.3 ± 0.5 | ND | ND |
| AG701i50-H242G | + | + | 0.8 ± 0.1 | 1.7 ± 0.2 | ND | ND |
a Each value is the mean of four independent measurements ± SD. ND, Not done.