Table II.
Targeted Protein Structure | Targeted Amino Acids | Primer Sequence (5′ to 3′)a | Resulting Amino Acids |
---|---|---|---|
Arabinose-5-P-binding site | R61T62 | GACAAGGCTAATGGAGGCTCGTCTAAATCATTCC | A61A62 |
GGAATGATTTAGACGAGCCTCCATTAGCCTTGTC | |||
PEP-binding site | K136 | GGAAAAGTTATTAACATTGGGAAGGGCC | G136 |
GGCCCTTCCCAATGTTAATAACTTTTCC | |||
Maintenance of active sites | C164 | GTCATGGTGGCTGAGAGAGG | A164 |
CCTCTCTCAGCCACCATGAC | |||
Maintenance of active sites | H198 | CTGATATAACTGGTTCATTACAAC | G198 |
GTTGTAATGAACCAGTTATATCAG | |||
Maintenance of active sites | H242 | ATGGAGGTGGGCAATGATCC | G242 |
GGATCATTGCCCACCTCCAT |
a Nucleotides introducing site-directed mutations in the coding sequence of LekdsA are underlined.