Table II.
Sets of oligonucleotides used for the site-directed mutagenesis of tomato Kdo-8P synthase encoded by pLEKdoS
| Targeted Protein Structure | Targeted Amino Acids | Primer Sequence (5′ to 3′)a | Resulting Amino Acids |
|---|---|---|---|
| Arabinose-5-P-binding site | R61T62 | GACAAGGCTAATGGAGGCTCGTCTAAATCATTCC | A61A62 |
| GGAATGATTTAGACGAGCCTCCATTAGCCTTGTC | |||
| PEP-binding site | K136 | GGAAAAGTTATTAACATTGGGAAGGGCC | G136 |
| GGCCCTTCCCAATGTTAATAACTTTTCC | |||
| Maintenance of active sites | C164 | GTCATGGTGGCTGAGAGAGG | A164 |
| CCTCTCTCAGCCACCATGAC | |||
| Maintenance of active sites | H198 | CTGATATAACTGGTTCATTACAAC | G198 |
| GTTGTAATGAACCAGTTATATCAG | |||
| Maintenance of active sites | H242 | ATGGAGGTGGGCAATGATCC | G242 |
| GGATCATTGCCCACCTCCAT |
a Nucleotides introducing site-directed mutations in the coding sequence of LekdsA are underlined.