Figure 7.

Hydrolysis of a leaf extract at pH 5.6 was measured using Z-Arg-Arg-pNA, Bz-Asn-pNA, and equistatin as substrates in the presence/absence of the indicated inhibitors. control, No inhibitor; K, kininogen domain 3; A, stefin A; C, cystatin C; P, potato cystatin. Error bars represent the se of the mean from four simultaneous measurements. The proteolysis of equistatin was measured by western-blot analysis. The amount of disappearance of intact protein in the absence of inhibitors (equistatin in preheated extract minus equistatin in plant extract) was taken as 100% proteolysis.