Figure 2.

Preference for DNA_HM vs. unmethylated DNA. Reaction mixtures of 50 μl contained 250 nM CcrM preincubated with 5 μM unmethylated or DNA_HM and initiated by the addition of 50 μM AdoMet. Data with DNA_HM were fit to the equation, y = Ae^−kt + Bx + C, where A is the burst amplitude, k is the observed rate constant for the burst amplitude, B is the steady state rate, and C is a defined constant. Data with unmethylated DNA were fit to the equation, y = Ae^−kt + C, where A is the burst amplitude, k is the observed rate constant for the burst amplitude, and C is a defined constant.