Table 1.

Catalytic constants of Ab 4f4f and Ab 24⁸ for various substrates

Antibody	Substrate	kcat, min−1	Km, μM	kcat/kuncat, × 10−5	kcat/Km, M−1⋅min−1
Ab 4f4f	p-Nitrophenyl β-d-glucopyranoside (3)	2.8  ×  10−3	22	2.0	130
Ab 24	p-Nitrophenyl β-d-glucopyranoside (3)	3.2  ×  10−4	160	0.25	1.7
Ab 4f4f	p-Nitrophenyl α-d-glucopyranoside (4)	6.2  ×  10−4	170	2.5	3.7
Ab 4f4f	p-Nitrophenyl β-d-galactopyranoside (5)	3.0  ×  10−3	190	1.1	16
Ab 4f4f	p-Nitrophenyl α-d-galactopyranoside (6)	1.2  ×  10−3	430	2.0	2.8
Ab 4f4f	p-Nitrophenyl β-d-fucopyranoside (7)	5.3  ×  10−4	80	1.0	6.7
Ab 4f4f	p-Nitrophenyl β-d-N-acetylglucosamide (8)	2.0  ×  10−2	110	0.21	180

k_uncat value is k′_H2O.