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EF-G is not a substrate for Lit-mediated cleavage. Purified EF-G and EF-Tu (5 μM) were each incubated with Lit protein with or without Gol peptide (both 5 μM) at 37°C for 1 hr as described in the Materials and Methods. Samples were subjected to SDS/PAGE (10%) and stained with Coomassie blue. The Lit protein has run off the gel so it is not visible.
