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. 1998 Mar 17;95(6):2896–2901. doi: 10.1073/pnas.95.6.2896

Figure 1.

Figure 1

Sulfotyrosine analysis. Microsomal extract (○) or buffer (•) was combined with PSGL-1 peptide-derivitized beads in the presence of [35S]PAPS and incubated under standard assay conditions. The beads were washed extensively and then treated with proteinase K. Released material was hydrolyzed under alkaline conditions and analyzed by HPLC.