Table 1.

Purification of rat liver TPST

Step	Protein			Sulfotransferase
	Volume, ml	Concentration, mg/ml	Total, mg	Total activity, units	Specific activity, units/mg	Yield, %	Purification, fold
Postnuclear superanant	4,060	34.6	140,400	23,080	0.16	—	—
Microsomal extract	3,720	11.6	43,000	36,510	0.85	100	5.3
Toyopearl SP-550C	300	12.6	3,780	8,880	2.35	24	14.7
Peptide column 1	60	0.38	23	4,600	200	13	1,250
Peptide column 2	30	0.026	0.8	2,360	2,950	8	18,440