Figure 2.

Determining the stability of initiation complexes of rrnB P1 with wild-type (WT) and the mutant RNAPs. The experiment was performed with 50 mM KCl in the presence of competitive DNA pKK223–3 (≈80 nM) as described in Materials and Methods. Note different time scales for different experiments. The controls without the inhibitor are indicated as −. (A) Autoradiogram of the transcripts. (B) The transcription activities from rrnB P1 and RNAI were plotted as function of time (after inhibitor addition).