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. 1998 Mar 17;95(6):2914–2919. doi: 10.1073/pnas.95.6.2914

Figure 2.

Figure 2

Na+-sugar cotransporters homologous to SGLT1 were tested for their capability to transport [14C]β-d-Glc-IPM. Xenopus laevis oocytes were injected with 50 nl of water with or without 10 ng cRNA of SGLT1 (rabbit), SGLT1 (man), Hu14 (man), SMIT (dog), or SAAT1 (pig). After 3–6 days of incubation, the expression of the respective transporter was controlled by measuring the phlorizin-inhibitable uptake after 30 min of incubation with 50 μM [14C]AMG (SGLT1s, SAAT1), 1.25 mM [14C]AMG (Hu14), and 1 μM [3H]myo-inositol (SMIT). The employed phlorizin concentrations were 100 μM (SGLT1 from rabbit), 200 μM (SGLT1 from man, Hu14, SAAT1), or 500 μM (SMIT). The expressed phlorizin-inhibitable uptake rates of AMG and myo-inositol were 274 ± 22 (SGLT1, rabbit), 48 ± 5 (SGLT1, man), 25 ± 1 (Hu14), 6.5 ± 0.7 (SMIT), and 18 ± 2 (SAAT1) pmol × oocyte−1 × h−1, respectively. Under identical experimental conditions the same batches of injected oocytes were tested for phlorizin-inhibitable uptake of 100 μM [14C]β-d-Glc-IPM. Medians and SEM values from 8–10 parallel determinations without and with phlorizin are indicated.