Figure 6.

Transcription of SAAT1 in human tumor cells. Total RNA was isolated from differentially graded human renal carcinomas (lane c, nuclear grade-1 tumor; lane d, nuclear grade-2 tumor; lane e, nuclear grade-3 tumor), from mouse tumor xenografts (lane f, human colon carcinoma; lane g, human ovary carcinoma), and from the human carcinoma cell lines KTCTL30 (lane h), KTCTL104 (lane i), or T84 (lane k). The RNAs and a control without RNA (lane b) were reverse transcribed, and PCRs were performed with primers that were specific for the cloned fragment of the human SAAT1. The amplification products, size markers, and the control were separated on an agarose gel and stained with ethidium bromide (Upper) or hybridized with an internal primer specific for the human SAAT1 (Lower).