Figure 7.

[¹⁴C]β-d-Glc-IPM uptake into suspended human carcinoma T84 cells, which is mediated by a phlorizin-sensitive transporter. (a) The time course of the phlorizin-inhibitable uptake of 0.2 mM [¹⁴C]β-d-Glc-IPM measured in the presence of sodium. (b) The substrate dependence of the initial phlorizin-inhibitable uptake rate of [¹⁴C]β-d-Glc-IPM, which was measured in the presence of Na⁺. Mean values ± SEM from four separate determinations are shown. In the presence of 0.5 mM phlorizin, nonspecific uptake of [¹⁴C]β-d-Glc-IPM was measured, which increased linearly with time and substrate concentration. The nonspecific uptake rate of [¹⁴C]β-d-Glc-IPM was 73 ± 2 pmol × mg ⁻¹ mM⁻¹ min⁻¹.