Figure 3.
Comparison of Tf/Tg (•) and Tf/Tc (▪) ratios averaged over the 10 training proteins. The colors represent different iterations of the EF γ values used to calculate the ratios. All of the ratios evaluated with the γ values obtained from the GSW optimization procedure (5) are shown in black. We calculated the average ratios with respect to each set of molten globule ensembles. The −1.0 mark represents the molten globule ensemble generated by translation, the 0.0 mark represents the ensembles generated with the 0th iterate γ values, etc. The plot reveals that the second iterate γ values produce the highest Tf/Tg on average and that the Tf/Tc ratio is being conserved through each round of optimization. For the second iterate optimization λ1 = −0.1 and λ2 = 78.0.