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Controls. (A) Addition of calcium to rigor had no significant effect on the spectrum. (B) Partially extracted fibers, which are activated by ATP with or without calcium, show essentially the same spectra as unextracted fibers that have been Ca-activated. On addition of vanadate, the overall distribution shifts toward that of relaxation. (C) Addition of ADP to rigor and (D) addition of vanadate to relaxation had little effect on the original spectrum.
