Figure 1.

Repression of Bcl-2 protein expression in HeLa/Tet-Bcl-2 cells by Tet. Western blot analysis was performed on the HeLa/Tet-Bcl-2 cell system to ensure adequate suppression of Bcl-2 protein. Protein samples were collected from cell populations that were cultured in parallel on chamber slides for microscopy. Cells were placed in culture with or with out 2 μg/ml Tet (lanes 2 and 1, respectively) and incubated for 48 h to regulate Bcl-2 protein expression. Coimmunodetection of actin was performed to assure equal loading.