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Quantitation of GSH in isolated nuclei from HeLa/Tet-Bcl-2 cells
| GSH
|
|||||
|---|---|---|---|---|---|
| Cell total
|
Nucleus
|
||||
| HeLa | pg/cell | mM | pg/nucleus | mM | % nuclear |
| Bcl-2 off | 5.4 ± 0.84 | 16 ± 2.4 | 1.7 ± 0.36 | 6 ± 1.3 | 32 |
| Bcl-2 on | 5.8 ± 0.95 | 14 ± 2.3 | 4.3 ± 1.51 | 16 ± 5.4 | 74 |
GSH content was calculated on a cell (nucleus) basis and reported as amount and concentration. Reported values are from three samples, and the standard deviation is reported. Similar results were observed in multiple experiments.
