Figure 1.

GFP-MR transcriptional activity is comparable to wild-type MR. CV-1 cells were transiently transfected with the reporter plasmid TAT3-LUC containing three near-consensus hormone response elements upstream of a minimal promoter driving expression of the luciferase gene. Expression vectors for GFP-MR or wild-type MR were cotransfected. Cells were incubated for 16 hr with 2 nM aldosterone at 30°C, as indicated. Shown is the average of four separate transfections (±SEM) performed on the same day. Comparable results were obtained in three independent experiments performed on different days: the ratio of GFP-MR activity to wild-type MR activity varied by less than 15%.