Figure 2.

Reverse transcriptase–PCR-based analyses of gene expression in cultured HL-1 cells. (A) α-MHC and β-MHC, (B) ANF, (C) α-skeletal actin (α-skeletal), (D) α-cardiac actin (α-cardiac), and (E) connexin43 (C43) in controls and cultured HL-1 cells at passage 86. Total RNA was isolated from AT-1 cells (AT-1), HL-1 cells (HL-1), ventricular cardiac muscle tissue from 17 day embryonic rat (e17), atrial muscle tissue from adult mouse (MA), ventricular cardiac muscle tissue from adult mouse (MV), and skeletal muscle tissue from adult mouse (MS). DNA molecular weight markers [φX174 RF DNA, HaeIII cut] (M).