Skip to main content
. 1998 Mar 17;95(6):2985–2990. doi: 10.1073/pnas.95.6.2985

Figure 1.

Figure 1

MCP-1-induced desensitization of the CCR2B receptor. Calcium influx was promoted by the addition of 10 nM human MCP-1 to Mono Mac 1 cells loaded with Fluo-3; a second challenge with 10 nM MCP-1 promoted very little calcium response (Upper). As a control, MCP-1-induced cells were stimulated with 10 nM SDF-1α (Lower). Calcium influx in response to MCP-1 was determined by flow cytometry at 525 nm, as detailed in the text. Data are given as a percentage of maximum MCP-1 fluorescence response. Arrows depict the time of chemokine addition.