Figure 5.

βARK-K220R inhibition of MCP-1-induced desensitization of the CCR2B receptor. HEK293 cells were transiently cotransfected with pcDNACCR2B and either empty vector or pcDNAβARK-K220R. Cells were stimulated first with different concentrations of MCP-1 as indicated. After approximately 2 min, 10 nM MCP-1 was added a second time as indicated by the arrows. Calcium was determined as described in the legend of Fig. 1. Data are given as a percentage of maximal MCP-1-induced and are representative of two independent experiments.