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. 1993 Jun;67(6):1268–1273. doi: 10.1038/bjc.1993.237

Fibroblast stimulation of breast cancer cell growth in a serum-free system.

M C Ryan 1, D J Orr 1, K Horgan 1
PMCID: PMC1968494  PMID: 8512812

Abstract

Conditioned media from 14 short term fibroblast cell lines were mitogenic for human breast cancer cells with different steroid receptor profiles in serum-free culture. Fibroblast-conditioned medium stimulated tritiated thymidine incorporation in short term culture and growth in a longer proliferation study as measured by the MTT colorimetric assay. Conditioned media from benign and malignant epithelial cells were non-stimulatory for breast cancer cells but that derived from endothelial cells showed similar stimulation to fibroblasts. Partial purification of fibroblast-conditioned medium identified a peptide with a molecular weight of approximately 8 kDa that showed no affinity for heparin and was mitogenic for MCF-7 breast cancer cells.

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Selected References

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