Figure 3.

Pharmacological characterization of prostaglandin effects on human spermatozoa. (A) Human sperm were loaded with the fluorescent dye fluo-3/AM and stimulated with different concentrations of PGE₁ (•), PGE₂ (▾). Sulprostone (⧫) was tested at 0.1, 1, and 10 μM and iloprost (○) was tested at 1 and 10 μM. Agonist-induced increases in [Ca²⁺]_i are indicated. Data represent means of three experiments. (B) HEL cells were loaded with fluo-3/AM and stimulated with agonists used in A. Agonist-induced rises in [Ca²⁺]_i were recorded. Iloprost (○) was tested at a concentration of 10 μM only. Data represent means of three experiments.