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Agonist-induced acrosomal exocytosis in capacitated human sperm. Capacitated human spermatozoa were incubated for 30 min in the presence of modulators as indicated. Mastoparan (50 μM) and the Ca2+ ionophore A23187 (10 μM) served as positive controls. All of the other agents were applied at 1-μM concentrations. The percentage of acrosome-reacted sperm is indicated. Two hundred sperm were evaluated per single assay. Data represent means ± SEM (n = 16–32) of four independent experiments.
