Figure 5.

Effect of capacitation and Zn²⁺ on agonist-induced AR. Swim-up human sperm were capacitated for 2 (A), 4 (B), or 6 (C) h in BWW-FCS medium, incubated for 30 min with progesterone (1 μM), PGE₁ (1 μM), or a combination of the two agonists (application of agonist indicated by +), and the percentage of acrosome-reacted sperm was determined as outlined in the legend of Fig. 4. (D) After a 6-h capacitation period in BWW-FCS, sperm were washed and resuspended in BWW-chloride ions. (E) Sperm were treated as in D but were stimulated in the presence of 200 μM Zn²⁺. Data represent means ± SEM of four independent experiments each performed in quadruplicate.