Table 1. Label incorporation by crude extracts.
Strains
|
|||
---|---|---|---|
Incubation conditions | Wild type (A348) | glgP/pgm (A5130) | glgB polar (A1120) |
Unprimed initiation | 0.0038 | 0.053 | ND |
Unprimed elongation | 85 | 0.77 | ND |
Primed elongation | 717 | 262 | ND |
Reactions were carried out as described in Materials and Methods by using crude extracts as enzyme source. Activities are expressed as nmol of glucose·h-1 per mg of protein. ND, not detected.