Fig. 2.

Uptake of metal ions into X. laevis oocytes expressing DCT1, D124A mutant, and D124A/Q126D mutant. The uptake experiment was performed as described in Materials and Methods. (A) Metal ions uptake into DCT1-expressing oocytes. (B) Metal ions uptake into oocytes expressing the mutant D124A. (C) Metal ions uptake into oocytes expressing the double mutant D124A/Q126D. The uptake solution contained 100 mM NaCl, 2 mM KCl, 1 mM MgCl₂, 1 mM CaCl₂, 10 mM Hepes, 2 mM Mes, 2 mM ascorbic acid, and 7 μM of the indicated metal ion in its chloride form. The data for each bar represent means (after subtraction of the control values) + SEM (n = 15).